Waters
Waters
CSH (Charged Surface Hybrid) Technology
The 1.7 µm Charged Surface Hybrid (CSH™) particle is Waters third generation hybrid particle technology. Based on Waters Ethylene Bridged Hybrid [BEH] particle technology, CSH particles incorporate a low level surface charge, designed to improve sample loadability and peak asymmetry in low-ionic-strength mobile phases, while maintaining the mechanical and chemical stability inherent in BEH particle technology.
The advantages of CSH Technology include:
· Superior peak shape for basic compounds
· Increased loading capacity
· Rapid column equilibration after changing mobile-phase pH
· Improved batch-to-batch reproducibility
· Exceptional stability at low and high pH
CSH particle technology is also available in HPLC particle sizes [3.5 and 5 µm] in the XSelect™ family of HPLC columns enabling seamless transfer between HPLC and UPLC® Technology platforms.
CSH Technology dramatically improves virtually all facets of LC column performance in acidic, low ionic strength mobile phases that are commonly used in the chromatographic laboratory.
ACQUITY UPLC CSH C18
The ACQUITY UPLC® CSH™ C18 column overcomes the poor peak shape encountered with most modern C18 columns in low ionic strength mobile phases due to low loading capacity. With a controlled low level of a positive surface charge in acidic mobile phases, ACQUITY UPLC CSH C18 provides industry-leading peak shape for basic analytes in low pH mobile phases.
The increased and improved loadability possible with CSH Technology permits the separation, identification and quantitation of closely eluting impurities or degradants. In this example, a high concentration (0.5 mg/mL) of imipramine was repeatedly injected while the concentration of amitriptyline was increased with each injection. Columns: ACQUITY UPLC CSH C18, 2.1 x 50 mm, 1.7 μm or Kinetex™ C18, 2.1 x 50 mm, 1.7 µm; Mobile phase A: H2O; Mobile phase B: MeCN; Mobile phase C: 2% HCOOH in H2O; 25 to 35% A in 2 minutes, 35 – 95% B from 2 – 3 minutes, %C was held at a constant 5%; Injection volume: 5 μL; Sample diluent: H2O; Sample concentration: Imipramine: 0.5 mg/mL; Amitriptyline: As indicated [% of Imipramine]; Column temperature 40 °C; Detection: UV @ 254 nm; System: ACQUITY UPLC H-Class with ACQUITY UPLC PDA.
ACQUITY UPLC CSH Phenyl-Hexyl
The ACQUITY UPLC® CSH™ Phenyl-Hexyl column provides complementary selectivity compared to a straight chain alkyl C18 column, particularly for aromatic species.
With a controlled low level of a positive surface charge in acidic mobile phases, the ACQUITY UPLC CSH Phenyl-Hexyl column provides industry-leading peak shape for basic analytes in low pH mobile phases.
Fast separation of basic drugs on an ACQUITY UPLC CSH Phenyl-Hexyl column, 2.1 x 50 mm, 1.7 μm; Mobile phase A: 10 mM NH4COOH, pH 3; Mobile phase B: MeCN; 5 to 55% B linear in 3 minutes; Injection volume: 4 μL; Column temperature 30 °C; Detection: UV @ 260 nm; Analytes: (1) aminopyrazine; (2) pindolol; (3) quinine; (4) quinine impurity; (5) labetalol; (6) diltiazem impurity; (7) diltiazem; (8) verapamil; (9) amitriptyline; System: ACQUITY UPLC with ACQUITY UPLC PDA.
ACQUITY UPLC CSH Fluoro-Phenyl
One of the challenges in designing stationary phases with large selectivity differences is maintaining an acceptable level of batch-to-batch reproducibility. Many commercially available columns with unique selectivities suffer from significant variability, particularly phases bonded with a propyl fluoro phenyl (PFP) ligand.
The 1.7 µm ACQUITY UPLC® CSH™ Fluoro-Phenyl column overcomes this limitation due to the CSH Technology bonding process. This process not only yields exceptional batch-to-batch reproducibility, but sustained selectivity across particle sizes with its HPLC analog, XSelect™ CSH Fluoro-Phenyl [available in 3.5 and 5 µm particle sizes].
Reproducibility and scalability of ACQUITY UPLC CSH Fluoro-Phenyl [1.7 µm] and XSelect CSH Fluoro-Phenyl [3.5 and 5 µm]. Gradient separations on 2.1 x 50 mm columns containing nine different batches of CSH Technology, Fluoro-Phenyl representing three [1.7, 3.5 and 5 µm] particle sizes; Gradient: A: 15.4 mM ammonium formate, pH 3; B: acetonitrile; 5 to 90% B linear in 5 minutes; Temperature: 30 °C; Injection volume: 5 µL; Detection: 254 nm; Flow rate: 0.5 mL/min; Analytes: [1] thiourea; [2] resorcinol; [3] metoprolol; [4] 3-nitrophenol; [5] 2-chlorobenzoic acid; [6] amitriptyline; [7] diethylphthalate; [8] fenoprofen; [9] dipropylphthalate; [10] pyrenesulfonic acid; System: ACQUITY UPLC.
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