YMC-Pack PROTEIN-RP

YMC-Pack PROTEIN-RP

◈ Product Information
YMC-Pack PROTEIN-RP is a reversed-phase column utilizing a silica gel base. It contains a stationary phase, specifically designed for separation of proteins or peptides. Problems that are associated with conventional reversed-phase columns with short alkyl chain lengths are minimized.
Robust column lifetime and excellent recovery of hydrophobic proteins are typically possible on this phase.

► Point
　• Improved recovery of proteins or peptides
　• Improved durability when used with TFA solution
　• Enabled elution of high molecular weight proteins

► Specification
　- Particle size :5㎛
　- Usable pH range :1.5-7.5

Improved recovery of proteins or peptides

Sample	Recovery (%)
	YMC-Pack PROTEIN-RP	C4 column, Company A	C4 column, Company B
Ribonuclease A	93	95	86
Cytochrome c	94	89	95
Lysozyme	98	93	76
Myoglobin	97	85	93
BSA	94	92	86
Ovalbumin	90	73	88
Transferrin	94	98	88
Insulin(bovine)	97	73	92
Insulin B chain	82	76	70
α-Mating factor	93	82	81
Leu-Enkephalin	92	84	91
Gly-Gly-Gly-Gly	95	86	70

Recovery of various proteins and peptides is shown. YMC-Pack PROTEIN-RP gives greater recovery than competitive C4 columns.

Improved durability when used with TFA solution

Flow conditions
• Eluent :water/TFA (100/0.1)
• Flow rate :1.0 mL/min
• Temperature :ambient

Measurement conditions
• Column :YMC-Pack PROTEIN-RP 250 x 4.6 mmI.D.
• Eluent :acetonitrile/water (40/60)
• Flow rate :1.0 mL/min
• Temperature :30ºC
• Detection :UV at 254 nm, 0.32AUFS








The graph shows the test result of the stability of the stationary phase with 0.1% aqueous TFA. Retention of diphenyl on C4 columns manufactured by other companies greatly decreases as time passes. This is caused by detachment of butyl groups from the packing material due to acid hydrolysis.
Retention of diphenyl on YMC-Pack PROTEIN-RP is shown to be stable after 500 hours of mobile phase flow.

◈ Ordering Information

YMC-Pack PROTEIN-RP Analytical Columns
Particle size(µm)	Column size Inner diameter x length(mm)	Product Number
S-5	4.6 X 150	PR99S05-1546WT
	4.6 X 250	PR99S05-2546WT
YMC-Pack PROTEIN-RP Semi-preparative Columns
Particle size(µm)	Column size Inner diameter x length(mm)	Product Number
S-5	10 X 150	PR99S05-2510WT
	20 X150	PR99S05-1520WT
	20 X 250	PR99S05-2520WT
YMC-Pack PROTEIN-RP Guard Cartridge Columns (Inner diameter 1.0,1.5,2.0mm:2-pack Inner diameter 4.0mm:3-pack)
Particle size(µm)	Column size Inner diameter x length(mm)	Product Number
S-5	4.0 X 23	PR99S05-G304CC

*When using it for the first time,please purchase the cartridge holder corresponding to inner diameter of column.

YMC-Pack PROTEIN-RP Cartridge Holder
		Product Number
Cartridge Holder (Inner diameter 4.0mm)		XPCHW
YMC-Pack PROTEIN-RP Guard Columns
Particle size(µm)	Column size Inner diameter x length(mm)	Product Number
S-5	4.0 X 10	PR99S05-0104WFG
	10 X 30	PR99S05-0310WTG
	20 X 50	PR99S05-0520WTG