MY MENU

YMC

제목

YMC-Pack PROTEIN-RP

작성자
예일테크
작성일
2008.06.17
첨부파일0
추천수
0
조회수
2914
내용

YMC-Pack PROTEIN-RP

◈ Product Information
YMC-Pack PROTEIN-RP is a reversed-phase column utilizing a silica gel base. It contains a stationary phase, specifically designed for separation of proteins or peptides. Problems that are associated with conventional reversed-phase columns with short alkyl chain lengths are minimized.
Robust column lifetime and excellent recovery of hydrophobic proteins are typically possible on this phase.

► Point
 • Improved recovery of proteins or peptides
 • Improved durability when used with TFA solution
 • Enabled elution of high molecular weight proteins

► Specification
 - Particle size :5㎛
 - Usable pH range :1.5-7.5

 Improved recovery of proteins or peptides


Sample

Recovery (%)

YMC-Pack
PROTEIN-RP

C4 column,
Company A

C4 column,
Company B

Ribonuclease A

93

95

86

Cytochrome c

94

89

95

Lysozyme

98

93

76

Myoglobin

97

85

93

BSA

94

92

86

Ovalbumin

90

73

88

Transferrin

94

98

88

Insulin(bovine)

97

73

92

Insulin B chain

82

76

70

α-Mating factor 

93

82

81

Leu-Enkephalin

92

84

91

Gly-Gly-Gly-Gly

95

86

70


Recovery of various proteins and peptides is shown. YMC-Pack PROTEIN-RP gives greater recovery than competitive C4 columns.

 Improved durability when used with TFA solution



Flow conditions
• Eluent :water/TFA (100/0.1)
• Flow rate :1.0 mL/min
• Temperature :ambient

Measurement conditions
• Column :YMC-Pack PROTEIN-RP 250 x 4.6 mmI.D.
• Eluent :acetonitrile/water (40/60)
• Flow rate :1.0 mL/min
• Temperature :30ºC
• Detection :UV at 254 nm, 0.32AUFS








The graph shows the test result of the stability of the stationary phase with 0.1% aqueous TFA. Retention of diphenyl on C4 columns manufactured by other companies greatly decreases as time passes. This is caused by detachment of butyl groups from the packing material due to acid hydrolysis.
Retention of diphenyl on YMC-Pack PROTEIN-RP is shown to be stable after 500 hours of mobile phase flow.

◈ Ordering Information
YMC-Pack PROTEIN-RP Analytical Columns

Particle size(µm)

Column size Inner diameter

x length(mm)

Product Number

S-5

4.6 X 150

PR99S05-1546WT

4.6 X 250

PR99S05-2546WT

YMC-Pack PROTEIN-RP Semi-preparative Columns

Particle size(µm)

Column size Inner diameter
x length(mm)

Product Number

S-5

10 X 150

PR99S05-2510WT

20 X150

PR99S05-1520WT

20 X 250

PR99S05-2520WT

YMC-Pack PROTEIN-RP Guard Cartridge Columns
(Inner diameter 1.0,1.5,2.0mm:2-pack Inner diameter 4.0mm:3-pack)

Particle size(µm)

Column size Inner diameter
x length(mm)

Product Number

S-5

4.0 X 23

PR99S05-G304CC

*When using it for the first time,please purchase the cartridge holder corresponding to inner diameter of column.
YMC-Pack PROTEIN-RP Cartridge Holder

 

Product Number

Cartridge Holder (Inner diameter 4.0mm)

XPCHW

YMC-Pack PROTEIN-RP Guard Columns

Particle size(µm)

Column size Inner diameter
x length(mm)

Product Number

S-5

4.0 X 10

PR99S05-0104WFG

10 X 30

PR99S05-0310WTG

20 X 50

PR99S05-0520WTG

0
0

게시물수정

게시물 수정을 위해 비밀번호를 입력해주세요.

댓글삭제게시물삭제

게시물 삭제를 위해 비밀번호를 입력해주세요.