YMC-Pack Polyamine II

YMC-Pack Polyamine II

◈ Product Information
YMC-Pack Polyamine II is a silica-based packing bonded with polyamine. It is particularly useful for separation of sugars. The column life of YMC-Pack Polyamine II in aqueous mobile phase is longer than conventional silica-based amino columns, and thus is applicable to separation of oligosaccharides using mobile phase with relatively higher water content. In addition, YMC-Pack Polyamine II can be used to separate ionic compounds with a combination of normal-phase mode and weak anion exchange mode.

► Point
　• Silica gel chemically bonded to polyamine
　• The most suitable column for separation of sugars
　• Solves durability problem of conventional silica-based amino columns
　• Useful for separation of hydrophilic compounds including vitamins
　• Useful for separation of fat-soluble compounds using nonaqueous mobile phase

► Specification
　- Particle size :5㎛
　- Pore size :120Å
　- Usable pH range :2.0-7.5

Excellent durability

Flow conditions
• Column :YMC-Pack Polyamine II　250 x 4.6mmI.D.
• Eluent :acetonitrile/water
• Flow Rate :1.0mL/min
• Temperature :ambient
• Time :100 hours

Analytical conditions
• Eluent :acetonitrile/water(75/25)
• Flow Rate :1.0mL/min
　　　　　　　　　　　　　 • Temperature :26ºC
　　　　　　　　　　　　　 • Detection :RI, 32 x 10-6 RIU/FS
Even in a hard flow durability test using mobile phase that consists only of water,the retention time of sugars changes little.

The most suitable column for separation of sugars

1.Fructose
2.Glucose
3.Sucrose
4.Maltose
5.Lactose

• Column :YMC-Pack Polyamine II, 250 x 4.6mmI.D.
• Eluent :acetonitrile/water (75/25)
• Flow Rate :1.0mL/min
• Temperature :26ºC
• Detection :RI, 32 x 10-6 RIU/FS

 

 

 

 

 

For normal-phase separation

• Column :YMC-Pack Polyamine II 250 x 4.6mmI.D.
• Eluent :n-hexane/ethyl acetate(70/30)
• Flow Rate :1.0mL/min
• Temperature :30ºC
• Detection :UV at 295 nm

 

 

 

 

 

 

 

• Column :YMC-Pack Polyamine II 250 x 4.6mmI.D.
• Eluent :50mM NH4H2PO4/acetonitrile(30/70)
• Flow Rate :1.0mL/min
• Temperature :30ºC
• Detection :UV at 250 nm



YMC-Pack Polyamine II is applicable for separation of fat-soluble vitamins and water-soluble vitamins as a normal-phase column that can be used with water or buffer and various organic solvents.

 

 

 

◈ Ordering Information

YMC-Pack Polyamine II Analytical Columns
Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
S-5	120	4.6 X 150	PB12S05-1546WT
		4.6 X 250	PB12S05-2546WT
		6.0 X 150	PB12S05-1506WT
		6.0 X 250	PB12S05-2506WT
YMC-Pack Polyamine II Semi-preparative Columns
Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
S-5	120	10 X 250	PB12S05-2510WT
		20 X 250	PB12S05-2520WT
YMC-Pack Polyamine II Guard Cartridge Columns (Inner diameter 1.0,1.5,2.0mm:2-pack Inner diameter 4.0mm:3-pack)
Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
S-5	120	4.0 X 23	PB12S05-G304CC

*When using it for the first time,please purchase the cartridge holder corresponding to inner diameter of column.

YMC-Pack Polyamine II Cartridge Holder
			Product Number
Cartridge Holder (Inner diameter 4.0mm)			XPCHW
YMC-Pack Polyamine II Guard Columns
Particle size (µm)	Pore size (Å)	Column size Inner diameter x length (mm)	Product Number
S-5	120	4.0 X 10	PB12S05-0104WFG
		5.0 X 10	PB12S05-0105WFG
		10 X 30	PB12S05-0310WTG
		20 X 50	PB12S05-0520WTG