YMC

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24 YMC-Pack PROTEIN-RP
YMC-Pack PROTEIN-RP

◈ Product Information
YMC-Pack PROTEIN-RP is a reversed-phase column utilizing a silica gel base. It contains a stationary phase, specifically designed for separation of proteins or peptides. Problems that are associated with conventional reversed-phase columns with short alkyl chain lengths are minimized.
Robust column lifetime and excellent recovery of hydrophobic proteins are typically possible on this phase.

► Point
　• Improved recovery of proteins or peptides
　• Improved durability when used with TFA solution
　• Enabled elution of high molecular weight proteins

► Specification
　- Particle size :5㎛
　- Usable pH range :1.5-7.5

　Improved recovery of proteins or peptides
Sample Recovery (%) YMC-Pack
PROTEIN-RP C4 column,
Company A C4 column,
Company B Ribonuclease A 93 95 86 Cytochrome c 94 89 95 Lysozyme 98 93 76 Myoglobin 97 85 93 BSA 94 92 86 Ovalbumin 90 73 88 Transferrin 94 98 88 Insulin(bovine) 97 73 92 Insulin B chain 82 76 70 α-Mating factor　 93 82 81 Leu-Enkephalin 92 84 91 Gly-Gly-Gly-Gly 95 86 70
Recovery of various proteins and peptides is shown. YMC-Pack PROTEIN-RP gives greater recovery than competitive C4 columns.

　Improved durability when used with TFA solution

Flow conditions
• Eluent :water/TFA (100/0.1)
• Flow rate :1.0 mL/min
• Temperature :ambient

Measurement conditions
• Column :YMC-Pack PROTEIN-RP 250 x 4.6 mmI.D.
• Eluent :acetonitrile/water (40/60)
• Flow rate :1.0 mL/min
• Temperature :30ºC
• Detection :UV at 254 nm, 0.32AUFS

The graph shows the test result of the stability of the stationary phase with 0.1% aqueous TFA. Retention of diphenyl on C4 columns manufactured by other companies greatly decreases as time passes. This is caused by detachment of butyl groups from the packing material due to acid hydrolysis.
Retention of diphenyl on YMC-Pack PROTEIN-RP is shown to be stable after 500 hours of mobile phase flow.

◈ Ordering Information YMC-Pack PROTEIN-RP Analytical Columns Particle size(µm) Column size Inner diameter x length(mm) Product Number S-5 4.6 X 150 PR99S05-1546WT 4.6 X 250 PR99S05-2546WT YMC-Pack PROTEIN-RP Semi-preparative Columns Particle size(µm) Column size Inner diameter
x length(mm) Product Number S-5 10 X 150 PR99S05-2510WT 20 X150 PR99S05-1520WT 20 X 250 PR99S05-2520WT YMC-Pack PROTEIN-RP Guard Cartridge Columns
(Inner diameter 1.0,1.5,2.0mm:2-pack Inner diameter 4.0mm:3-pack) Particle size(µm) Column size Inner diameter
x length(mm) Product Number S-5 4.0 X 23 PR99S05-G304CC *When using it for the first time,please purchase the cartridge holder corresponding to inner diameter of column. YMC-Pack PROTEIN-RP Cartridge Holder Product Number Cartridge Holder (Inner diameter 4.0mm) XPCHW YMC-Pack PROTEIN-RP Guard Columns Particle size(µm) Column size Inner diameter
x length(mm) Product Number S-5 4.0 X 10 PR99S05-0104WFG 10 X 30 PR99S05-0310WTG 20 X 50 PR99S05-0520WTG 예일테크 2008.06.17

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23 YMC-Pack Diol-SEC
YMC-Pack Diol-SEC ◈ Product Information YMC-Pack Diol-SEC is a size exclusion chromatography column utilizing a silica gel base. Diol-120, 200, and 300 are suitable for separation or molecular weight determination of proteins with molecular weights of 10,000 to several hundred thousands. Diol-60 is the most suitable for separation of peptides of oligosaccharides whose molecular weights are 10,000 or less. ► Point 　 • Utilizes silica gel base with high resistance to pressure 　 • Low-cost gel filtration column 　 • Useful for molecular weight determination of proteins and sugars 　 • Minimal secondary interactions ► Specification 　 - Particle size :5㎛　 - Pore size :60A, 120A, 200A, 300A 　 - Usable pH range :5.0-7.5 　Calibration curves of proteins obtained using columns with different pore sizes 1. Glutamate dehydrogenase 2. Lactate dehydrogenase 3. Enolase 4. Adenylate kinase 5. Cytochrome c • Column :YMC-Pack Diol-SEC 500 x 8.0 mmI.D. • Eluent :0.1M KH2PO4-K2HPO4 (pH 7.0) containing 0.2M NaCl • Flow rate :0.7 mL/min • Temperature :ambient • Detection :UV at 280 nm Diol-120,Diol-200,and Diol-300 are suitable for the separation or molecular weight determination of proteins with molecular weights of 10,000 to several hundred thousands. 　For separation of proteins with molecular weights of 10,000 to several hundred thousands 1.Glutamate dehydrogenase(MW 290,000) 2. Lactate dehydrogenase(MW 142,000) 3. Enolase(MW 67,000) 4. Adenylate kinase(MW 32,000) 5. Cytochrome c(MW 12,400) • Column :YMC-Pack Diol-SEC　500 x 8.0 mmI.D. • Eluent :0.1M KH2PO4-K2HPO4 (pH 7.0) containing 0.2M NaCl • Flow rate :0.7 mL/min • Temperature :ambient • Detection :UV at 280 nm 　For separation of peptides with molecular weights of 10,000 or less 1.Insulin (Bovine) 2.Neurotensin 3.Angiotensin-II 4.Glycine • Column :YMC-Pack Diol-60 500 x 8.0 mmI.D. • Eluent :0.1M KH2PO4-K2HPO4(pH 7.0) containing 0.2MNaCl /acetonitrile(70/30) • Flow rate :0.7 mL/min • Temperature :ambient • Detection :UV at 215 nm 1. Myoglobin-17,000(MW) 2. Ribonuclease A-13,700(MW) 3. Cytochrome c-12,400(MW) 4. Insulin (Bovine)-5,700(MW) 5. Insulin B chain-3,496(MW) 6. α-Mating factor-1,684(MW) 7. Neurotensin-1,672(MW) 8. Angiotensin-I-1,296(MW) 9. CCK-octapeptide-1,143(MW) 10. Bradykinin-1,060(MW) 11. Angiotensin-II-1,040(MW) 12. Oxytocin-1,007(MW) 13. Angiotensin-III-931(MW) 14. Met-enkephalin-573(MW) 15. Leu-enkephalin-555(MW) 16. Tetraglycine-246(MW) 17. Glycine-75(MW) 　For molecular weight determination of sugars For separation or molecular weight determination of neutral sugars and water-soluble high-molecular-weight compounds,Diol-60,Diol-120,Diol-200,and Diol-300 are useful individually or in combination. ◈ Ordering Information YMC-Pack Diol-SEC Analysis Columns(Stainless Columns) Packing material Column size Inner diameter x length(mm) Product Number Diol-60 8.0×300 DL06S05-3008WT 8.0×500 DL06S05-5008WT Diol-120 8.0×300 DL12S05-3008WT 8.0×500 DL12S05-5008WT Diol-200 8.0×300 DL20S05-3008WT 8.0×500 DL20S05-5008WT Diol-300 8.0×300 DL30S05-3008WT 8.0×500 DL30S05-5008WT YMC-Pack Diol-SEC Guard Columns(Stainless Columns) Packing material Column size Inner diameter x length(mm) Product Number Diol-60 8.0×30 DL06S05-0308WTG Diol-120 8.0×30 DL12S05-0308WTG Diol-200 8.0×30 DL20S05-0308WTG Diol-300 8.0×30 DL30S05-0308WTG YMC-Pack Diol-SEC Analysis Columns(Glass Columns) Packing material Column size Inner diameter x length(mm) Product Number Diol-60 8.0×300 DL06S05-3008FG 8.0×500 DL06S05-5008FG Diol-120 8.0×300 DL12S05-3008FG 8.0×500 DL12S05-5008FG Diol-200 8.0×300 DL20S05-3008FG 8.0×500 DL20S05-5008FG Diol-300 8.0×300 DL30S05-3008FG 8.0×500 DL30S05-5008FG 예일테크 2008.06.17

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22 YMC CHIRAL PREP CD ST/PM
YMC CHIRAL PREP CD ST/PM

◈ Product Information
YMC CHIRAL PREP CD ST/PM are suitable packing materials for preparative isolation of optical isomers. ST is suitable for use mainly in reversed-phase mode, while PM can be used in both normal-phase and reversed-phase modes. The cost is considerably less than that of the conventional packing materials for optical isomers, and therefore YMC CHIRAL PREP CD ST/PM are appropriate not only for semi-preparative isolation but also for industrial isolation. They are also applicable to simulated moving bed(SMB) chromatography and axial compression columns.

► Point
　• β-cyclodextrin modified silica gel packing material
　• Can separate various optical isomers
　• Can be used in both normal-phase and reversed-phase modes
　• Applicable to large-scale isolation by simulated moving bed(SMB) chromatography
　• Various particle sizes are available
　• Bulk supply at kilogram scale is available
　• Excellent cost performance

► Specification
　- Particle size :10㎛, 20㎛, 50㎛
　- Pore size :120A
　- Usable pH range :2.0-7.0

　For preparative isolation of optical isomers
Column :YMC CHIRAL PREP CD ST(10µm, 120Å)
　
Scale up from analytical to preparative conditions is fast and easy with high sample loading.

　High isolation performance

Column :YMC CHIRAL PREP CD ST(10µm, 120Å)
　
Scale up from analytical to preparative conditions is fast and easy with high sample loading.
　Optical isomer 　Column 　Load* 　Optical purity
(F1) 　Recovery
amount(F1) 　Recovery
rate(F1) 1. PM 100mg >99.9% 49.6mg 99.2% 2. ST 100mg >99.9% 47.7mg 94.8% 3. ST 100mg >99.9% 38.5mg 77.0% 4. PM 200mg >99.9% 96.5mg 96.5% 4. PM 250mg >99.9% 114.2mg 91.3%
*Column size :250X20mm I.D.　(10µm, 120Å)
　
Highly pure optical isomers can be obtained with high recovery.

　Can be used in both normal-phase and reversed-phase modes

• Column :YMC CHIRAL PREP CD PM(10µm,120Å) 250X4.6 mm I.D.
• Eluent :hexane/ethanol(95/5)
• Flow rate :0.5mL/min
• Temperature :ambient
• Detection :UV at 254 nm
• Injection :5µL(1mg/mL)

• Column :YMC CHIRAL PREP CD ST (10µm,120Å) 250X4.6 mm I.D.
• Eluent :20mM KH2PO4(pH4.6) /acetonitrile(99/1)
• Flow rate :1.0mL/min
• Temperature :ambient
• Detection :UV at 220 nm
• Injection :5µL(1mg/mL)

Besides water, alcohol or acetonitrile, hexane or tetrahydrofuran can be used as mobile phase.

◈ Ordering Information YMC CHIRAL PREP CD ST/PM Analytical Columns Packing
material Particle size
(µm) Pore size (Å) Column size Inner diameter x length(mm) Product Number ST S-10 120 4.6 X 250 ST12S11-2546WT PM 4.6 X 250 PM12S11-2546WT YMC CHIRAL PREP CD ST/PM Packing material Product name Particle size
(µm) Pore size(Å) Product Number YMC CHIRAL PREP CD ST 10 120 ST12S11 20 ST12S21 30 ST12S50 YMC CHIRAL PREP CD PM 10 PM12S11 20 PM12S21 30 PM12S50 예일테크 2008.06.17

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21 YMC CHIRAL NEA
YMC CHIRAL NEA

◈ Product Information
YMC CHIRAL NEA(R) and (S) phase optical isomer separation columns. Chiral discrimination is based on the higher-order structure of chiral macromolecules, which includes hydrogen bonds, π-π interaction, hydrophobic interaction, etc.

► Point
　• Synthesized macromolecule-type chiral column
　• Elution order can be reversed by selecting (R) or (S)
　• Excellent cost performance

► Specification
　- Particle size :5㎛
　- Usable pH range :2.0-6.5

　Used with reversed phase eluent

◈ Ordering Information YMC CHIRAL NEA Analytical Columns for Reversed-phase YMC*Gel Particle size
(µm) Pore size(Å) Column size Inner diameter
x length(mm) Product Number NEA(R) S-5 300 4.6 X 150 NR30S05-1546WT 4.6 X 250 NR30S05-2546WT NEA(S) S-5 300 4.6 X 150 NS30S05-1546WT 4.6 X 250 NS30S05-2546WT YMC CHIRAL NEA Analytical Columns for Normal-phase YMC*Gel Particle size
(µm) Pore size(Å) Column size Inner diameter
x length(mm) Product Number NEA(R) S-5 300 4.6 X 150 CR30S05-1546WT 4.6 X 250 CR30S05-2546WT NEA(S) S-5 300 4.6 X 150 CS30S05-1546WT 4.6 X 250 CS30S05-2546WT YMC CHIRAL NEA Guard Cartridge Columns for Reversed-phase
(Inner diameter 1.0,1.5,2.0mm:2-pack Inner diameter 4.0mm:3-pack) YMC*Gel Particle size
(µm) Pore size(Å) Column size Inner diameter
x length(mm) Product Number NEA(R) S-5 300 4.0 X 23 NR30S05-G304CC NEA(S) S-5 300 4.0 X 23 NS30S05-G304CC *When using it for the first time,please purchase the cartridge holder corresponding to inner diameter of column. YMC CHIRAL NEA Guard Cartridge Columns for Normal-phase
(Inner diameter 1.0,1.5,2.0mm:2-pack Inner diameter 4.0mm:3-pack) YMC*Gel Particle size
(µm) Pore size(Å) Column size Inner diameter
x length(mm) Product Number NEA(R) S-5 300 4.0 X 23 CR30S05-G304CC NEA(S) S-5 300 4.0 X 23 CS30S05-G304CC *When using it for the first time,please purchase the cartridge holder corresponding to inner diameter of column. YMC NEA Cartridge Holder Product Number Cartridge Holder (Inner diameter 4.0mm) XPCHW 예일테크 2008.06.17

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20 YMC CHIRAL CD BR Series
YMC CHIRAL CD BR Series

◈ Product Information
YMC CHRAL CD BR series is composed of 3 types of optical isomer separation columns. Each column possesses α-,β-,or γ-bromo-cyclodextrin as a functional group. Selection from the 3 types of columns enables analysis of a wide range of compounds. In addition, YMC CHIRAL CD BR series shows different selectivity from ODS because the separation is based on host-guest interaction. YMC CHIRAL CD BR series is useful for separating structural isomers that are difficult to separate using ODS.

► Specification
　- Particle size :5㎛
　- Pore size :120Å
　- Usable pH range :3.5-6.5

　Column 　YMC CHIRAL α-CD BR
　250 x 4.6mmI.D. 　YMC CHIRAL β-CD BR
　250 x 4.6mmI.D. 　YMC CHIRAL γ-CD BR
　250 x 4.6mmI.D. 　Eluent 　0.1M CH3COOH-CH3COONa (pH4.0)/ methanol(90/10) 　methanol/water (20/80) 　methanol/water (50/50) 　Flow rate 　1.0mL/min 　1.0mL/min 　1.0mL/min 　Temperature 　25ºC 　25ºC 　30ºC 　Detection 　UV at 254 nm 　UV at 254 nm 　UV at 230 nm
α-CD BR,β-CD BR,γ-CD BR show different separation feature because each of them has different size of cavities. Therefore choosing proper one can result in providing a successful separation of a wide-range compounds. It is also effective to use together with ODS columns.

　Column 　YMC CHIRAL β-CD BR
　250 x 4.6mmI.D. 　YMC CHIRAL β-CD BR
　250 x 4.6mmI.D. 　YMC CHIRAL γ-CD BR
　250 x 4.6mmI.D. 　Eluent 　0.5M triethylamine-
　acetic acid
　(pH4.0)/
　acetonitrile(10/90) 　0.5M triethylamine-
　acetic acid
　(pH5.4)/
　acetonitrile(95/5) 　0.5M triethylamine-
　acetic acid
　(pH4.0)/
　acetonitrile(60/40) 　Flow rate 　1.0mL/min 　1.0mL/min 　1.0mL/min 　Temperature 　25ºC 　25ºC 　25ºC 　Detection 　UV at 254 nm 　UV at 254 nm 　UV at 230 nm
YMC CHIRAL CD BR series provide better separation for various optical isomers because cyclodextrin is a chiral compound. YMC CHIRAL CD BR series is effective as a chiral column for reversed phase eluent.

◈ Ordering Information YMC CHIRAL CD BR Series Analytical Columns Packing
material Particle size
(µm) Pore size
(Å) Column size Inner diameter
x length(mm) Product Number α-CD BR S-5 120 4.6 X 150 DA12S05-1546WT 4.6 X 250 DA12S05-2546WT β-CD BR S-5 120 4.6 X 150 DB12S05-1546WT 4.6 X 250 DB12S05-2546WT γ-CD BR S-5 120 4.6 X 150 DG12S05-1546WT 4.6 X 250 DG12S05-2546WT YMC CHIRAL CD BR Series Guard Cartridge Columns
(Inner diameter 1.0,1.5,2.0mm:2-pack Inner diameter 4.0mm:3-pack) Packing
material Particle size
(µm) Pore size
(Å) Column size Inner diameter
x length(mm) Product Number α-CD BR S-5 120 4.0 X 23 DA12S05-G304CC β-CD BR S-5 120 4.0 X 23 DB12S05-G304CC γ-CD BR S-5 120 4.0 X 23 DG12S05-G304CC *When using it for the first time,please purchase the cartridge holder corresponding to inner diameter of column. YMC CHIRAL CD BR Series Cartridge Holder Product Number Cartridge Holder (Inner Diameter 4.0mm) XPCHW 예일테크 2008.06.17

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19 YMC-Pack PA
YMC-Pack PA

◈ Product Information
YMC-Pack PA is useful for separation of acidic oligosaccharides.

► Point
　• Silica gel chemically bonded with polyamine

► Specification
　- Particle size :5㎛
　- Pore size :120Å
　- Usable pH range :4.0-7.5

◈ Ordering Information YMC-Pack PA Analytical Columns Particle size
(µm) Pore size
(Å) Column size Inner diameter
x length (mm) Product Number S-5 120 4.6 X 150 PA12S05-1546WT 4.6 X 250 PA12S05-2546WT YMC-Pack PA Guard Columns Particle size
(µm) Pore size
(Å) Column size Inner diameter
x length (mm) Product Number S-5 120 4.0 X 10 PA12S05-0104WFG 예일테크 2008.06.16

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18 YMC-Pack NH2
YMC-Pack NH2

◈ Product Information
YMC-Pack NH2 is a normal-phase separation column utilizing the polarity of primary amino groups. It is also applicable to separations utilizing weak anion exchange. YMC-Pack NH2 is often used for separation of sugars.

► Point
　• Silica gel chemically bonded with aminopropyl group
　• Useful for separation of sugars
　• Enables normal-phase mode separation using aqueous or nonaqueous mobile phase

► Specification
　- Particle size :5㎛
　- Pore size :120Å
　- Usable pH range :2.0-7.5

◈ Ordering Information YMC-Pack NH2 Analytical Columns Particle size
(µm) Pore size
(Å) Column size Inner diameter x length
(mm) Product Number S-5 120 4.6 X 100 NH12S05-1046WT 4.6 X 150 NH12S05-1546WT 4.6 X 250 NH12S05-2546WT 4.6 X 300 NH12S05-3046WT 6.0 X 100 NH12S05-1006WT 6.0 X 150 NH12S05-1506WT 6.0 X 250 NH12S05-2506WT 6.0 X 300 NH12S05-3006WT YMC-Pack NH2 Semi-preparative Columns Particle size
(µm) Pore size
(Å) Column size Inner diameter x length
(mm) Product Number S-5 120 10 X 150 NH12S05-1510WT 10 X 250 NH12S05-2510WT 10 X 300 NH12S05-3010WT 20 X 150 NH12S05-1520WT 20 X 250 NH12S05-2520WT YMC-Pack NH2 Guard Cartridge Columns
(Inner diameter 1.0,1.5,2.0mm:2-pack Inner diameter 4.0mm:3-pack) Particle size
(µm) Pore size
(Å) Column size Inner diameter x length
(mm) Product Number S-5 120 4.0 X 23 NH12S05-G304CC *When using it for the first time,please purchase the cartridge holder corresponding to inner diameter of column. YMC-Pack NH2 Cartridge Holder Product Number Cartridge Holder (Inner diameter 4.0mm) XPCHW YMC-Pack NH2 Guard Columns Particle size
(µm) Pore size
(Å) Column size Inner diameter x length
(mm) Product Number S-5 120 4.0 X 10 NH12S05-0104WFG 5.0 X 10 NH12S05-0105WFG 10 X 30 NH12S05-0310WTG 20 X 50 NH12S05-0520WTG 예일테크 2008.06.16

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17 YMC-Pack Polyamine II
YMC-Pack Polyamine II

◈ Product Information
YMC-Pack Polyamine II is a silica-based packing bonded with polyamine. It is particularly useful for separation of sugars. The column life of YMC-Pack Polyamine II in aqueous mobile phase is longer than conventional silica-based amino columns, and thus is applicable to separation of oligosaccharides using mobile phase with relatively higher water content. In addition, YMC-Pack Polyamine II can be used to separate ionic compounds with a combination of normal-phase mode and weak anion exchange mode.

► Point
　• Silica gel chemically bonded to polyamine
　• The most suitable column for separation of sugars
　• Solves durability problem of conventional silica-based amino columns
　• Useful for separation of hydrophilic compounds including vitamins
　• Useful for separation of fat-soluble compounds using nonaqueous mobile phase

► Specification
　- Particle size :5㎛
　- Pore size :120Å
　- Usable pH range :2.0-7.5

　Excellent durability
Flow conditions
• Column :YMC-Pack Polyamine II　250 x 4.6mmI.D.
• Eluent :acetonitrile/water
• Flow Rate :1.0mL/min
• Temperature :ambient
• Time :100 hours

Analytical conditions
• Eluent :acetonitrile/water(75/25)
• Flow Rate :1.0mL/min
　　　　　　　　　　　　　 • Temperature :26ºC
　　　　　　　　　　　　　 • Detection :RI, 32 x 10-6 RIU/FS
Even in a hard flow durability test using mobile phase that consists only of water,the retention time of sugars changes little. 　The most suitable column for separation of sugars
1.Fructose
2.Glucose
3.Sucrose
4.Maltose
5.Lactose

• Column :YMC-Pack Polyamine II, 250 x 4.6mmI.D.
• Eluent :acetonitrile/water (75/25)
• Flow Rate :1.0mL/min
• Temperature :26ºC
• Detection :RI, 32 x 10-6 RIU/FS

　For normal-phase separation

• Column :YMC-Pack Polyamine II 250 x 4.6mmI.D.
• Eluent :n-hexane/ethyl acetate(70/30)
• Flow Rate :1.0mL/min
• Temperature :30ºC
• Detection :UV at 295 nm

• Column :YMC-Pack Polyamine II 250 x 4.6mmI.D.
• Eluent :50mM NH4H2PO4/acetonitrile(30/70)
• Flow Rate :1.0mL/min
• Temperature :30ºC
• Detection :UV at 250 nm

YMC-Pack Polyamine II is applicable for separation of fat-soluble vitamins and water-soluble vitamins as a normal-phase column that can be used with water or buffer and various organic solvents.

◈ Ordering Information YMC-Pack Polyamine II Analytical Columns Particle size
(µm) Pore size
(Å) Column size Inner diameter x length
(mm) Product Number S-5 120 4.6 X 150 PB12S05-1546WT 4.6 X 250 PB12S05-2546WT 6.0 X 150 PB12S05-1506WT 6.0 X 250 PB12S05-2506WT YMC-Pack Polyamine II Semi-preparative Columns Particle size
(µm) Pore size
(Å) Column size Inner diameter x length
(mm) Product Number S-5 120 10 X 250 PB12S05-2510WT 20 X 250 PB12S05-2520WT YMC-Pack Polyamine II Guard Cartridge Columns
(Inner diameter 1.0,1.5,2.0mm:2-pack Inner diameter 4.0mm:3-pack) Particle size
(µm) Pore size
(Å) Column size Inner diameter
x length
(mm) Product Number S-5 120 4.0 X 23 PB12S05-G304CC *When using it for the first time,please purchase the cartridge holder corresponding to inner diameter of column. YMC-Pack Polyamine II Cartridge Holder Product Number Cartridge Holder (Inner diameter 4.0mm) XPCHW YMC-Pack Polyamine II Guard Columns Particle size
(µm) Pore size
(Å) Column size Inner diameter x length
(mm) Product Number S-5 120 4.0 X 10 PB12S05-0104WFG 5.0 X 10 PB12S05-0105WFG 10 X 30 PB12S05-0310WTG 20 X 50 PB12S05-0520WTG 예일테크 2008.06.16

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16 YMC-Pack Diol-NP
YMC-Pack Diol-NP

◈ Product Information
A Normal Phase Silica Alternative
The YMC Diol-NP stationary phase is a versatile alternative to silica for normal phase separations. The bonded layer's-OH groups provide good selectivity without excessive retention, since hydrogen bonding with the Diol-NP layer is not as strong as one with the silanols on a bare silica surface.
Diol-NP columns also provide better reproducibility when compared with bare silica columns.

High Product Recovery
Like all YMC silica-based bonded phase, the Diol-NP phase starts with an underlying silica support of exceptional purity.
YMC manufacturing and quality assurance possession ensure that the silica has a very low metal content. The silica purity greatly reduces nonspecific sample adsorption. As a result, you can expect excellent sample recovery

High Preparative Throughput
The high surface area of the underlying silica particle, together with the larger number of available sites for interaction of the 1, 2 dihydroxypropane ligands, results in good preparative loading.

► Point
　• Improved peak shape versus bare silica
　• Good selectivity without excessive retention
　• High recovery of product
　• High prep loading
　• Bonded phase reproducibility
　• Long column life

► Specification
　- Particle size :5㎛
　- Pore size :60Å, 120Å
　- Usable pH range :2.0-7.5

　Separation feature of Diol-NP and SIL

1.Phenol
2.Catechol
3.Resorcinol
4.Hydroquinone
5.Pyrogallol
6.Phloroglucinol

• Column size :250 x 4.6mmI.D.
• Eluent :hexane/ethanol (80/20)
• Flow Rate :1.0mL/min
• Temperature :30 ºC
• Detection :UV at 254 nm

This figure shows differences in selectivity of phenols when separated on Diol-NP and SIL under the same conditions. For these compounds, Diol columns provide longer retention time and better separation.

◈ Ordering Information YMC-Pack Diol-NP Analytical Columns Packing
material Particle size
(µm) Pore size
(Å) Column size Inner diameter x length (mm) Product Number Diol-60 S-5 60 4.6 X 100 DN06S05-1046WT 4.6 X 150 DN06S05-1546WT 4.6 X 250 DN06S05-2546WT Diol-120 S-5 120 4.6 X 100 DN12S05-1046WT 4.6 X 150 DN12S05-1546WT 4.6 X 250 DN12S05-2546WT YMC-Pack Diol-NP Semi-preparative Columns Packing
material Particle size
(µm) Pore size
(Å) Column size Inner diameter x length
(mm) Product Number Diol-60 S-5 60 20 X 150 DN06S05-1520WT 20 X 250 DN06S05-2520WT Diol-120 S-5 120 20 X 150 DN12S05-1520WT 20 X 250 DN12S05-2520WT 예일테크 2008.06.16

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14 YMCbasic
YMCbasic

◈ Product Information
Designed for Analysis of Basic Compounds
YMCbasic is a unique reversed-phase column designed for analysis of pharmaceuticals and other basic compounds. YMCbasic utilizes a novel silica synthesis and state-of-art silane bonding to create a column which gives true reversed-phase character, high resolution and excellent peak shape for the analysis of basic compounds.

"Neutral" Silica Plus Maximum Bonding Coverage
The silica support used in YMCbasic is prepared by an advanced synthetic process which yields a chromatographic material with proprietary pore size and “neutral” silanols minimize interactions with both basic and acidic compounds. The stationary phase is a monomeric bonding of several different alkyl silanes with chain length of C8 and smaller.

Provides True Reversed-Phase Selectivity
The “neutral” silica support used in YMCbasic exhibits minimal secondary retention interactions so selectivity is true reversed-phase. There are no special surface treatments or exotic endcapping procedures. This means that YMCbasic provides the same general selectivity seen with conventional C18 and C8 columns without basic compounds tailing.

► Point
　• Designed for the analysis of basic compounds
　• Utilizes a “neutral” silica support
　• Secondary interactions minimized as much as possible
　• Useful for separation of peptides

► Specification
　- Particle size :3㎛, 5㎛
　- Pore size :wide pore
　- Carbon contents :7%
　- Usable pH range :2.0-7.5

◈ Ordering Information YMCbasic Analytical Columns Particle size
(µm) Column size Inner diameter x length
(mm) Product Number S-3 2.0 X 50 BA99S03-0502WT 2.0 X 100 BA99S03-1002WT 2.0 X 150 BA99S03-1502WT 3.0 X 50 BA99S03-0503WT 3.0 X 100 BA99S03-1003WT 3.0 X 150 BA99S03-1503WT 4.6 X 50 BA99S03-0546WT 4.6 X 100 BA99S03-1046WT 4.6 X 150 BA99S03-1546WT S-5 2.0 X 50 BA99S05-0502WT 2.0 X 100 BA99S05-1002WT 2.0 X 150 BA99S05-1502WT 2.0 X 250 BA99S05-2502WT 3.0 X 50 BA99S05-0503WT 3.0 X 100 BA99S05-1003WT 3.0 X 150 BA99S05-1503WT 3.0 X 250 BA99S05-2503WT 4.6 X 50 BA99S05-0546WT 4.6 X 100 BA99S05-1046WT 4.6 X 150 BA99S05-1546WT 4.6 X 250 BA99S05-2546WT YMCbasic Guard Cartridge Columns
(Inner diameter 1.0,1.5,2.0mm:2-pack Inner diameter 4.0mm:3-pack) Particle size
(µm) Column size Inner diameter x length
(mm) Product Number S-3 2.0 X 10 BA99S03-0102CC 4.0 X 23 BA99S03-G304CC S-5 2.0 X 10 BA99S05-0102CC 4.0 X 23 BA99S05-G304CC *When using it for the first time,please purchase the cartridge holder corresponding to inner diameter of column. YMCbasic Cartridge Holder Product Number Semi Micro Guard Cartridge Holder (Inner diameter 1.0,1.5,2.0mm) XPCHSMW Cartridge Holder (Inner diameter 4.0mm) XPCHW 예일테크 2008.06.16

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13 YMC-Pack CN
YMC-Pack CN

◈ Product Information
Most Polar Reversed-Phase Column
In a reversed-phase mode, Cyano columns are the most polar and least retentive of all reversed-phase packings.
Extremely hydrophobic compounds, which do not elute on standard C18 and C8 packings with typical reversed-phase eluents, can be chromatographed using Cyano columns.
In addition, the unique nitrile chemistry provides different selectivity from both phenyl and standard aliphatic (C18, C8 and C4) reversed-phase packing. Large pore size 300Å packing materials are also available for the analysis of biomolecules.

Alternative to Silica
In a normal phase mode, Cyano is a bonded phase alternative to silica. Bonded normal phase columns have the advantage of faster equilibration and more uniform surface activity than bare silica gel. To extend column life, continued switching back and forth between normal and reversed phase solvents should be avoided.

► Point
　• Useful in both normal and reversed-phase applications
　• Stable high coverage monomerically bonded phase
　• Fully endcapped
　• Available in spherical 3, 5, 10, 15 and 20µm
　• Analytical, semi-prep and preparative column sizes available

► Specification
　- Particle size :3㎛, 5㎛, 10㎛
　- Pore size :120A, 300A
　- Carbon contents :7%, 3%
　- Usable pH range :2.0-7.5

　Normal Phase mode - Reversed Phase mode

Useful in both normal and reversed-phase mode. Elution sequence is reversed by the difference of separation mode.

◈ Ordering Information YMC-Pack CN Analytical Columns Particle size
(µm) Pore size
(Å) Column size Inner diameter x length
(mm) Product Number S-3 120 2.0 X 35 CN12S03-H502WT 2.0 X 50 CN12S03-0502WT 2.0 X 75 CN12S03-L502WT 2.0 X 100 CN12S03-1002WT 2.0 X 150 CN12S03-1502WT 3.0 X 50 CN12S03-0503WT 3.0 X 100 CN12S03-1003WT 3.0 X 150 CN12S03-1503WT 4.6 X 100 CN12S03-1046WT 4.6 X 150 CN12S03-1546WT S-5 120 1.0 X 150 CN12S05-1501WT 1.0 X 250 CN12S05-2501WT 1.5 X 150 CN12S05-15P5WT 1.5 X 250 CN12S05-25P5WT 2.0 X 150 CN12S05-1502WT 2.0 X 250 CN12S05-2502WT 4.6 X 75 CN12S05-L546WT 4.6 X 100 CN12S05-1046WT 4.6 X 150 CN12S05-1546WT 4.6 X 250 CN12S05-2546WT 4.6 X 300 CN12S05-3046WT 6.0 X 100 CN12S05-1006WT 6.0 X 150 CN12S05-1506WT 6.0 X 250 CN12S05-2506WT 6.0 X 300 CN12S05-3006WT 300 1.0 X 150 CN30S05-1501WT 1.0 X 250 CN30S05-2501WT 1.5 X 150 CN30S05-15P5WT 1.5 X 250 CN30S05-25P5WT 2.0 X 150 CN30S05-1502WT 2.0 X 250 CN30S05-2502WT 4.6 X 75 CN30S05-L546WT 4.6 X 100 CN30S05-1046WT 4.6 X 150 CN30S05-1546WT 4.6 X 250 CN30S05-2546WT 4.6 X 300 CN30S05-3046WT 6.0 X 100 CN30S05-1006WT 6.0 X 150 CN30S05-1506WT 6.0 X 250 CN30S05-2506WT 6.0 X 300 CN30S05-3006WT S-10 120 4.6 X 100 CN12S11-1046WT 4.6 X 150 CN12S11-1546WT 4.6 X 250 CN12S11-2546WT 4.6 X 300 CN12S11-3046WT 6.0 X 100 CN12S11-1006WT 6.0 X 150 CN12S11-1506WT 6.0 X 250 CN12S11-2506WT 6.0 X 300 CN12S11-3006WT YMC-Pack CN Semi-preparative Columns Particle size
(µm) Pore size
(Å) Column size Inner diameter x length
(mm) Product Number S-5 120 10 X 150 CN12S05-1510WT 10 X 250 CN12S05-2510WT 10 X 300 CN12S05-3010WT 20 X 100 CN12S05-1020WT 20 X 150 CN12S05-1520WT 20 X 250 CN12S05-2520WT 300 20 X 150 CN30S05-1520WT 20 X 250 CN30S05-2520WT S-10 120 10 X 150 CN12S11-1510WT 10 X 250 CN12S11-2510WT 10 X 300 CN12S11-3010WT 20 X 150 CN12S11-1520WT 20 X 250 CN12S11-2520WT YMC-Pack CN Guard Cartridge Columns
(Inner diameter 1.0,1.5,2.0mm:2-pack Inner diameter 4.0mm:3-pack) Particle size
(µm) Pore size
(Å) Column size Inner diameter
x length (mm) Product Number S-3 120 2.0 X 10 CN12S03-0102CC 4.0 X 23 CN12S03-G304CC S-5 120 1.0 X 10 CN12S05-0101CC 1.5 X 10 CN12S05-01P5CC 2.0 X 10 CN12S05-0102CC 4.0 X 23 CN12S05-G304CC 300 1.0 X 10 CN30S05-0101CC 1.5 X 10 CN30S05-01P5CC 2.0 X 10 CN30S05-0102CC 4.0 X 23 CN30S05-G304CC *When using it for the first time,please purchase the cartridge holder corresponding to inner diameter of column. YMC-Pack CN Cartridge Holder Product Number Semi Micro Guard Cartridge Holder(Inner diameter 1.0,1.5,2.0mm) XPCHSMW Cartridge Holder (Inner diameter 4.0mm) XPCHW YMC-Pack CN Guard Columns Particle size
(µm) Pore size
(Å) Column size Inner diameter x length
(mm) Product Number S-5 120 4.0 X 10 CN12S05-0104WFG 5.0 X 10 CN12S05-0105WFG 10 X 30 CN12S05-0310WTG 20 X 50 CN12S05-0520WTG 300 4.0 X 10 CN30S05-0104WFG 5.0 X 10 CN30S05-0105WFG 10 X 30 CN30S05-0310WTG 20 X 50 CN30S05-0520WTG S-10 120 4.0 X 10 CN12S11-0104WFG 5.0 X 10 CN12S11-0105WFG 10 X 30 CN12S11-0310WTG 20 X 50 CN12S11-0520WTG 예일테크 2008.06.16

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