YMC
YMC-Pack Diol-SEC
◈ Product Information
YMC-Pack Diol-SEC is a size exclusion chromatography column utilizing a silica gel base. Diol-120, 200, and 300 are suitable for separation or molecular weight determination of proteins with molecular weights of 10,000 to several hundred thousands. Diol-60 is the most suitable for separation of peptides of oligosaccharides whose molecular weights are 10,000 or less.
► Point
• Utilizes silica gel base with high resistance to pressure
• Low-cost gel filtration column
• Useful for molecular weight determination of proteins and sugars
• Minimal secondary interactions
► Specification
- Particle size :5㎛
- Pore size :60A, 120A, 200A, 300A
- Usable pH range :5.0-7.5
|
Calibration curves of proteins obtained using columns with different pore sizes |
1. Glutamate dehydrogenase
2. Lactate dehydrogenase
3. Enolase
4. Adenylate kinase
5. Cytochrome c
• Column :YMC-Pack Diol-SEC 500 x 8.0 mmI.D.
• Eluent :0.1M KH2PO4-K2HPO4 (pH 7.0) containing 0.2M NaCl
• Flow rate :0.7 mL/min
• Temperature :ambient
• Detection :UV at 280 nm
Diol-120,Diol-200,and Diol-300 are suitable for the separation or molecular weight determination of proteins with molecular weights of 10,000 to several hundred thousands.
|
For separation of proteins with molecular weights of 10,000 to several hundred thousands |
1.Glutamate dehydrogenase(MW 290,000)
2. Lactate dehydrogenase(MW 142,000)
3. Enolase(MW 67,000)
4. Adenylate kinase(MW 32,000)
5. Cytochrome c(MW 12,400)
• Column :YMC-Pack Diol-SEC 500 x 8.0 mmI.D.
• Eluent :0.1M KH2PO4-K2HPO4 (pH 7.0) containing 0.2M NaCl
• Flow rate :0.7 mL/min
• Temperature :ambient
• Detection :UV at 280 nm
|
For separation of peptides with molecular weights of 10,000 or less |
1.Insulin (Bovine)
2.Neurotensin
3.Angiotensin-II
4.Glycine
• Column :YMC-Pack Diol-60 500 x 8.0 mmI.D.
• Eluent :0.1M KH2PO4-K2HPO4(pH 7.0) containing 0.2MNaCl /acetonitrile(70/30)
• Flow rate :0.7 mL/min
• Temperature :ambient
• Detection :UV at 215 nm
1. Myoglobin-17,000(MW)
2. Ribonuclease A-13,700(MW)
3. Cytochrome c-12,400(MW)
4. Insulin (Bovine)-5,700(MW)
5. Insulin B chain-3,496(MW)
6. α-Mating factor-1,684(MW)
7. Neurotensin-1,672(MW)
8. Angiotensin-I-1,296(MW)
9. CCK-octapeptide-1,143(MW)
10. Bradykinin-1,060(MW)
11. Angiotensin-II-1,040(MW)
12. Oxytocin-1,007(MW)
13. Angiotensin-III-931(MW)
14. Met-enkephalin-573(MW)
15. Leu-enkephalin-555(MW)
16. Tetraglycine-246(MW)
17. Glycine-75(MW)
|
For molecular weight determination of sugars |
For separation or molecular weight determination of neutral sugars and water-soluble high-molecular-weight compounds,Diol-60,Diol-120,Diol-200,and Diol-300 are useful individually or in combination.
◈ Ordering Information
|
YMC-Pack Diol-SEC Analysis Columns(Stainless Columns) | ||
|
Packing material |
Column size Inner diameter x length(mm) |
Product Number |
|
Diol-60 |
8.0×300 |
DL06S05-3008WT |
|
8.0×500 |
DL06S05-5008WT | |
|
Diol-120 |
8.0×300 |
DL12S05-3008WT |
|
8.0×500 |
DL12S05-5008WT | |
|
Diol-200 |
8.0×300 |
DL20S05-3008WT |
|
8.0×500 |
DL20S05-5008WT | |
|
Diol-300 |
8.0×300 |
DL30S05-3008WT |
|
8.0×500 |
DL30S05-5008WT | |
|
YMC-Pack Diol-SEC Guard Columns(Stainless Columns) | ||
|
Packing material |
Column size Inner diameter x length(mm) |
Product Number |
|
Diol-60 |
8.0×30 |
DL06S05-0308WTG |
|
Diol-120 |
8.0×30 |
DL12S05-0308WTG |
|
Diol-200 |
8.0×30 |
DL20S05-0308WTG |
|
Diol-300 |
8.0×30 |
DL30S05-0308WTG |
|
YMC-Pack Diol-SEC Analysis Columns(Glass Columns) | ||
|
Packing material |
Column size Inner diameter x length(mm) |
Product Number |
|
Diol-60 |
8.0×300 |
DL06S05-3008FG |
|
8.0×500 |
DL06S05-5008FG | |
|
Diol-120 |
8.0×300 |
DL12S05-3008FG |
|
8.0×500 |
DL12S05-5008FG | |
|
Diol-200 |
8.0×300 |
DL20S05-3008FG |
|
8.0×500 |
DL20S05-5008FG | |
|
Diol-300 |
8.0×300 |
DL30S05-3008FG |
|
8.0×500 |
DL30S05-5008FG | |
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