YMC
YMC-Pack Diol-SEC
◈ Product Information
YMC-Pack Diol-SEC is a size exclusion chromatography column utilizing a silica gel base. Diol-120, 200, and 300 are suitable for separation or molecular weight determination of proteins with molecular weights of 10,000 to several hundred thousands. Diol-60 is the most suitable for separation of peptides of oligosaccharides whose molecular weights are 10,000 or less.
► Point
• Utilizes silica gel base with high resistance to pressure
• Low-cost gel filtration column
• Useful for molecular weight determination of proteins and sugars
• Minimal secondary interactions
► Specification
- Particle size :5㎛
- Pore size :60A, 120A, 200A, 300A
- Usable pH range :5.0-7.5
Calibration curves of proteins obtained using columns with different pore sizes |
1. Glutamate dehydrogenase
2. Lactate dehydrogenase
3. Enolase
4. Adenylate kinase
5. Cytochrome c
• Column :YMC-Pack Diol-SEC 500 x 8.0 mmI.D.
• Eluent :0.1M KH2PO4-K2HPO4 (pH 7.0) containing 0.2M NaCl
• Flow rate :0.7 mL/min
• Temperature :ambient
• Detection :UV at 280 nm
Diol-120,Diol-200,and Diol-300 are suitable for the separation or molecular weight determination of proteins with molecular weights of 10,000 to several hundred thousands.
For separation of proteins with molecular weights of 10,000 to several hundred thousands |
1.Glutamate dehydrogenase(MW 290,000)
2. Lactate dehydrogenase(MW 142,000)
3. Enolase(MW 67,000)
4. Adenylate kinase(MW 32,000)
5. Cytochrome c(MW 12,400)
• Column :YMC-Pack Diol-SEC 500 x 8.0 mmI.D.
• Eluent :0.1M KH2PO4-K2HPO4 (pH 7.0) containing 0.2M NaCl
• Flow rate :0.7 mL/min
• Temperature :ambient
• Detection :UV at 280 nm
For separation of peptides with molecular weights of 10,000 or less |
1.Insulin (Bovine)
2.Neurotensin
3.Angiotensin-II
4.Glycine
• Column :YMC-Pack Diol-60 500 x 8.0 mmI.D.
• Eluent :0.1M KH2PO4-K2HPO4(pH 7.0) containing 0.2MNaCl /acetonitrile(70/30)
• Flow rate :0.7 mL/min
• Temperature :ambient
• Detection :UV at 215 nm
1. Myoglobin-17,000(MW)
2. Ribonuclease A-13,700(MW)
3. Cytochrome c-12,400(MW)
4. Insulin (Bovine)-5,700(MW)
5. Insulin B chain-3,496(MW)
6. α-Mating factor-1,684(MW)
7. Neurotensin-1,672(MW)
8. Angiotensin-I-1,296(MW)
9. CCK-octapeptide-1,143(MW)
10. Bradykinin-1,060(MW)
11. Angiotensin-II-1,040(MW)
12. Oxytocin-1,007(MW)
13. Angiotensin-III-931(MW)
14. Met-enkephalin-573(MW)
15. Leu-enkephalin-555(MW)
16. Tetraglycine-246(MW)
17. Glycine-75(MW)
For molecular weight determination of sugars |
For separation or molecular weight determination of neutral sugars and water-soluble high-molecular-weight compounds,Diol-60,Diol-120,Diol-200,and Diol-300 are useful individually or in combination.
◈ Ordering Information
YMC-Pack Diol-SEC Analysis Columns(Stainless Columns) | ||
Packing material |
Column size Inner diameter x length(mm) |
Product Number |
Diol-60 |
8.0×300 |
DL06S05-3008WT |
8.0×500 |
DL06S05-5008WT | |
Diol-120 |
8.0×300 |
DL12S05-3008WT |
8.0×500 |
DL12S05-5008WT | |
Diol-200 |
8.0×300 |
DL20S05-3008WT |
8.0×500 |
DL20S05-5008WT | |
Diol-300 |
8.0×300 |
DL30S05-3008WT |
8.0×500 |
DL30S05-5008WT | |
YMC-Pack Diol-SEC Guard Columns(Stainless Columns) | ||
Packing material |
Column size Inner diameter x length(mm) |
Product Number |
Diol-60 |
8.0×30 |
DL06S05-0308WTG |
Diol-120 |
8.0×30 |
DL12S05-0308WTG |
Diol-200 |
8.0×30 |
DL20S05-0308WTG |
Diol-300 |
8.0×30 |
DL30S05-0308WTG |
YMC-Pack Diol-SEC Analysis Columns(Glass Columns) | ||
Packing material |
Column size Inner diameter x length(mm) |
Product Number |
Diol-60 |
8.0×300 |
DL06S05-3008FG |
8.0×500 |
DL06S05-5008FG | |
Diol-120 |
8.0×300 |
DL12S05-3008FG |
8.0×500 |
DL12S05-5008FG | |
Diol-200 |
8.0×300 |
DL20S05-3008FG |
8.0×500 |
DL20S05-5008FG | |
Diol-300 |
8.0×300 |
DL30S05-3008FG |
8.0×500 |
DL30S05-5008FG |
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